Soybean [Glycine max (L.) Merr].

نویسندگان

  • Diane Luth
  • Katey Warnberg
  • Kan Wang
چکیده

In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wounding for infection to occur. We have continued to make improvements in the protocol, resulting in an efficient plant regeneration system.

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عنوان ژورنال:
  • Methods in molecular biology

دوره 1223  شماره 

صفحات  -

تاریخ انتشار 2015